Inhibition of 1, 2-dimethylhydrazine-induced mucin-depleted foci and O⁶-methylguanine DNA adducts in the rat colorectum by boiled garlic powder.

摘要: The scavenging capacity of reactive oxygen species, such as hydroxyl radicals, is reported not to decrease in boiled garlic (an odorless preparation). We therefore examined the modifying effect powder (BGP) on 1,2-dimethylhydrazine-induced mucin-depleted foci (MDF) and aberrant crypt (ACF), preneoplastic lesions, rat colorectum. Male F344 rats (5 weeks old) were fed a basal diet, or experimental diets containing 5% 1% BGP for 5 weeks. One week later, all injected s.c. with DMH (40 mg/kg, once weekly 2 weeks). At 10 age, sacrificed, colorectum was evaluated MDF ACF. In given (Groups 3), numbers decreased significantly dose-dependent manner, compared diet value (Group 1) (p<0.01). ACF Group 2, but 3, showed non-significant tendency decrease. Next, effects formation DMH-induced O6-methylguanine (O6-MeG) DNA adducts studied. 10% All i.p. 40 mg/kg at end 5. animals sacrificed 6 hours after injection analyze O6-MeG colorectal mucosa. Dietary administration inhibited adduct levels mucosa, controls These results suggested that may exert chemopreventive against colon carcinogenesis least initiation stage.