A strong promoter element is located between alternative exons of a gene encoding the human gamma-aminobutyric acid-type A receptor beta 3 subunit (GABRB3).
作者： E.F. Kirkness , C.M. Fraser
DOI: 10.1016/S0021-9258(18)53626-7
关键词:
摘要: The gene that encodes the beta 3 subunit of gamma-aminobutyrate-Type A (GABAA) receptor is widely expressed in brain tissue and has been associated with imprinted genetic disorders. Here, 5' regions human rat genes were characterized found to be highly conserved both coding non-coding sequences. novel transcript revealed existence an alternative exon 1 (exon 1a) a variant signal sequence. Relative levels transcripts vary between fetal adult brain, different regions. Endogenous also detected several immortalized cell lines, including kidney 293 cells. Transcription initiated from multiple sites within pyrimidine-rich region gene. This exhibits strong promoter activity binds nuclear factors at site which overlaps transcriptional start sites. element was shown bind Sp1 least one other unidentified factor.
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