Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

作者: Frank B Dean , John R Nelson , Theresa L Giesler , Roger S Lasken

DOI: 10.1101/GR.180501

关键词:

摘要: … Random priming of M13 single-stranded DNA gave … was amplified 375-fold in 24 h by using random hexamers. … and heated in the presence of random hexamer primers, as described. …

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