Label-Free Hyperspectral Imaging of Intracellular Hemoglobin in Human Erythrocytes

摘要: A broadband hyperspectral imaging (HSI) technique, originally developed for remote sensing and in geophysical applications, recently has been employed to enable noninvasive label-free optical of biological specimens. Here we report our results on microscopy non-labeled erythrocytes study the distribution hemoglobin. We have chosen focus cellular hemoglobin because it is an endogenous chromorphore that present high concentrations, which makes a good candidate establishing proof principle. In data cubes (x, y, λ) are collected by either wide-field or confocal microscope equipped with light source, such as white laser spectral engine narrow-band selection wavelength scanning achieved fast optoelectronic devices. The obtained then analyzed algorithms based angle mapper, yielding set unique absorption signatures (i.e. endmembers) corresponding subforms hemoglobin, oxyhemoglobin methemoglobin. Wavelength-dependent scattering cell membranes also resolved. Unique endmembers specific identified used build map intracellular estimating abundance each from cube. Our ongoing efforts include technology development determination local thickness single erythrocyte, volumetric measurement. technique may be further molecular chemical broad range biomarkers cells tissues, ultimately vivo well.