Metabolism of gamma-hexachlorocyclohexane by Arthrobacter citreus strain BI-100: Identification of metabolites.

作者: Jharna Datta , Amiya K. Maiti , Deba P. Modak , Pran K. Chakrabartty , Prantosh Bhattacharyya

DOI: 10.2323/JGAM.46.59

关键词:

摘要: Growth characteristics of the aerobic bacterial strain Arthrobacter citreus BI-100 in mineral salts medium with gamma-hexachlorocyclohexane (gamma-HCH) as sole source carbon and degradation gamma-HCH by are reported. The highest yield bacteria is observed at a concentration 100 mg/L. At this concentration, entered exponential phase growth without any lag. 8 h growth, no residual HCH, but its metabolites, was detectable medium. bacterium attained stationary 48 72 h; metabolite could be detected gas chromatography. Six metabolic intermediates produced A. different periods were characterized using chromatography-mass spectrometry high-performance liquid chromatography, which furnished evidence for presence gamma-1,3,4,5,6-pentachlorocyclohexene, tetrachlorocyclohexene, trichlorocyclohexa-diene, 2-chlorophenol, phenol, catechol, among others.

参考文章(32)
Ryozo Imai, Yuji Nagata, Keishi Senoo, Hidenori Wada, Masao Fukuda, Masamichi Takagi, Keiji Yano, Dehydrochlorination of γ-Hexachlorocyclohexane (γ-BHC) by γ-BHC-Assimilating Pseudomonas paucimobilis Agricultural and biological chemistry. ,vol. 53, pp. 2015- 2017 ,(1989) , 10.1080/00021369.1989.10869597
A. J. Francis, R. J. Spanggord, G. I. Ouchi, Degradation of Lindane by Escherichia coli Applied and Environmental Microbiology. ,vol. 29, pp. 567- 568 ,(1975) , 10.1128/AM.29.4.567-568.1975
HERMAN J. BENEZET, F. MATSUMURA, Isomerization of γ-BHC to α-BHC in the Environment Nature. ,vol. 243, pp. 480- 481 ,(1973) , 10.1038/243480A0
Siddhartha K. Sahu, Kamala Kumari Patnaik, Manikonda Sharmila, N. Sethunathan, Degradation of Alpha-, Beta-, and Gamma-Hexachlorocyclohexane by a Soil Bacterium under Aerobic Conditions Applied and Environmental Microbiology. ,vol. 56, pp. 3620- 3622 ,(1990) , 10.1128/AEM.56.11.3620-3622.1990
A D Heritage, I C Rae, Identification of intermediates formed during the degradation of hexachlorocyclohexanes by Clostridium sphenoides. Applied and Environmental Microbiology. ,vol. 33, pp. 1295- 1297 ,(1977) , 10.1128/AEM.33.6.1295-1297.1977
A D Heritage, I C MacRae, Degradation of lindane by cell-free preparations of Clostridium sphenoides Applied and Environmental Microbiology. ,vol. 34, pp. 222- 224 ,(1977) , 10.1128/AEM.34.2.222-224.1977
T S Marks, J D Allpress, A Maule, Dehalogenation of lindane by a variety of porphyrins and corrins. Applied and Environmental Microbiology. ,vol. 55, pp. 1258- 1261 ,(1989) , 10.1128/AEM.55.5.1258-1261.1989