Multipotent mesenchymal stem cells reduce interstitial fibrosis but do not delay progression of chronic kidney disease in collagen4A3-deficient mice

作者: V. Ninichuk , O. Gross , S. Segerer , R. Hoffmann , E. Radomska

DOI: 10.1038/SJ.KI.5001521

关键词:

摘要: Multipotent mesenchymal stem or stromal cells (MSC) have shown to improve outcome of acute renal injury models, but whether MSC can delay failure in chronic kidney disease is not known. We injected primary saline into mice that lack the α3-chain type IV collagen (COL4A3), a model with close similarities human Alport disease. Weekly injections from week 6 10 life prevented loss peritubular capillaries and reduced markers fibrosis, is, interstitial volume, numbers smooth muscle actin-positive cells, deposits as compared saline-injected COL4A3-deficient mice. However, function, blood urea nitrogen, creatinine levels, proteinuria well survival were affected by injections. Although found localize kidneys after injection, differentiation was detected. expressed growth factors, vascular endothelial factor (VEGF) bone morphogenetic protein-7 under basal culture conditions. In fact, VEGF mRNA levels increased MSC-injected supernatants enhance cell proliferation vitro. Thus, weekly prevent possibly owing local production factors rather than cells. The maintenance vasculature associated less fibrosis but, insufficient

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