Compositions and methods for pathogen transport

摘要: This disclosure provides a method for transporting pathogen under ambient conditions, by culturing the with an amoeba conditions that favor incorporation of into trophozoite, starving until it encysts, then conversion back to trophozoite. In one aspect, cyst comprises contacting in iron rich environment. Virus and/or bacteria are pathogens can be transported disclosed method. Amoeba useful methods include, without limitation Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria gruberi. The have utility in: from military field hospitals clinics laboratory; global satellite laboratories clinical laboratories; long term storage pathogens; enriching contaminated patient samples interest; biosurveillance detection efforts.