Bioconversion of secondary fiber fines to ethanol using counter-current enzymatic saccharification and co-fermentation.

摘要: This research examined several enzymatic and microbial process for the conversion of waste cellulosic fibers into ethanol. The first was a one-stage in which pulp fines were contacted with commercial enzyme solutions. second used sequential, multistage saccharification. third sequential addition countercurrent mode. Experiments compared results various feedstocks, different enzymes, supplementation β-glucosidase, saccharification combined fermentation. highest (65%) from 4% consistency sugar concentration (5.4%) an 8% attained when 5 FPU/g plus 10 IU/g β-glucosidase used. Sequential to small aliquots produced higher overall yield/U than same total amount single dose. In fermentation experiments, we 2.12% ethanol 5.4% solution. represents 78% theoretical maximum. yield could probably be increased through optimization step. Even little occurred, facilitated separation water, fiber, ash, so cellulase treatment effective means dewatering sludges.