Physicochemical characterization of ten fractions of bovine alpha lipoproteins.

摘要: With the onset of milk production, serum concentrations alpha lipoproteins in dairy cow steadily increase, frequently attaining values greater than 1.5 g/dl. Since these comprise a highly polydisperse system, we have carried out studies to explore differences among bovine density interval between 1.05 1.21 g/ml. Separation into ten fractions was achieved ultracentrifugally an isopycnic gradient. Agarose gel electrophoresis showed that all but bottom fraction contained as either major or sole lipoprotein class. Compositional analyses revealed increasing percentage both protein and phospholipid decreasing cholesterol with density. The esterified unesterified ratio ranged from 3 8 top densities particles obtained various were calculated sedimentation velocity measurements compositional analyses. resulting agreed well solution gradient fractions. apoprotein each apoA-I. Combining diffusion coefficient data by intensity fluctuation spectroscopy data, able calculate molecular weights, frictional ratios, Einstein-Stokes radii for three Results are discussed terms previously published on other mammalian data.