Characteristics of hydrogen and bioflocculant production by a transposon-mutagenized strain of Pantoea agglomerans BH18
作者： Hongyan Liu , Hongyu Wang , Haihua Qin
DOI: 10.1016/J.IJHYDENE.2016.10.091
关键词:
摘要: Abstract Based on the transposon-mutagenized library of Pantoea agglomerans BH18, mutant strain 2-103 was obtained using screens for high simultaneous production hydrogen and bioflocculant. The able to grow in medium with 50 μg/mL kanamycin could be amplified Tn7-based transposon PCR amplification cells from several passages, indicating that insertion stable. cell culture batch experiments varying initial pH values, glucose concentrations NaCl determined. result indicated optimum bioflocculant activity 7.0 8.0, respectively. optimal concentration 20 g/L. In addition, produce under marine fresh conditions. had highest (1.55 ± 0.07) mol H2/mol (83.7 ± 5.89)% conditions, which increased (1.78 ± 0.08) (93.5 ± 6.24)% This study found has ability within a range concentrations, is beneficial improvement cost-effective bio-treatment processes.
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