Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance.

摘要: Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl and Pel that play distinct roles in attachment biofilm formation for monospecies biofilms. Considerably less is known about their role the development of mixed species communities. This study has investigated during P. a defined experimentally informative community, consisting aeruginosa, protegens Klebsiella pneumoniae. Loss polysaccharide had biggest impact on integration biofilms, where percent composition psl mutant was significantly lower (0.06%) than its wild-type parent (2.44%). In contrast, loss no development. alginate or overproduction resulted representing 8.4% 18.11%, respectively, biofilm. Dual biofilms K. pneumoniae were not affected by Psl, while mucoid strain achieved greater biomass strain. When grown with protegens, polysaccharides different from formed PAO1. comprised 35-40% which higher (5-20%). reduced percentage dual (<1%). disrupted communal stress resistance Thus, an individual impacts emergent properties such