Adoptive transfer of helminth antigen‐pulsed dendritic cells protects against the development of experimental colitis in mice

摘要: Infection with helminth parasites and treatment worm extracts can suppress inflammatory disease, including colitis. Postulating that dendritic cells (DCs) participated in the suppression of inflammation seeking to move beyond use helminths per se, we tested ability Hymenolepis diminuta antigen-pulsed DCs colitis as a novel cell-based immunotherapy. Bone marrow derived pulsed H. antigen (HD-DCs), or PBS-, BSA-, LPS-DCs controls, were transferred into wild-type (WT), interleukin-10 (IL-10) knock-out (KO), RAG-1 KO mice, impact on dinitrobenzene sulphonic acid (DNBS)-induced splenic cytokine production assessed 72 h later. Mice receiving HD-DCs significantly protected from DNBS-induced experimental groups only these mice displayed increased Th2 cytokines IL-10 production. Adoptive transfer neither nor DNBS-colitis. Furthermore, CD4(+) splenocytes recipients naive against DNBS-colitis, an dependent manner. Thus, are anti-colitic immunotherapy educate T cells: mechanistically, effect requires host has adaptive immune response mobilize IL-10.