Destination and consequences of Panx1 and mutant expression in polarized MDCK cells.

摘要: The channel-forming membrane glycoprotein pannexin 1 (Panx1) is best characterized as an ATP release channel. To investigate the trafficking and sorting of Panx1, we used polarized MDCK cells non-polarized BICR-M1Rk to track fate GFP-tagged Panx1. In cells, Panx1 was found throughout plasma membrane, including lamellipodia tumor cell surface-targeting domain mapped residues 307-379. preferentially enriched at apical grown monolayer sheets or spheroids. Residual localized within basolateral membranes independent a putative dileucine motif LL365/6 C-terminal Unexpectedly, stable expression mutant, where tyrosine-based (YxxO) mutated (Y308F), truncated Δ379 caused lose cell-cell contacts their ability polarize they underwent switch more fibroblast-like phenotype. We conclude that delivered epithelial mutants drive phenotypic changes preventing polarization.