Somatic hypermutation of a lambda 2 transgene under the control of the lambda enhancer or the heavy chain intron enhancer.

摘要: Previous experiments have suggested an important role for the Ig enhancers and transcription in targeting somatic hypermutation. To determine whether requirement of is chain specific, we analyzed two lambda 2 light transgenes under control different enhancers, either 2-4 enhancer or heavy intron enhancer. The were amplified cloned from B220+PNAhigh B cells Peyer's patches (PP) SRBC-immunized spleen. transgene underwent mutation both PP splenic cell populations, but at a frequency lower than endogenous genes. This could be replaced by Interestingly, enhancer-driven construct showed evidence statistically significant population, not spleen-derived population. difference hypermutation suggests that can more readily detected isolated PP. ability to replace shows locus specific. Given these very few elements common, our results further suggest are primarily timing mutating