Evaluation of a cocktail of three bacteriophages for the biocontrol of Salmonella of wastewater

摘要: article i nfo Article history: Received 27 January 2011 Accepted 31 May Salmonella serovars are increasing in importance as significant pathogens of both human and animals. Although water wastewater treated to eliminate pathogenic microorganisms, they still play an important role the transmission spp. In this study, bacteriophages infecting were isolated from evaluated; for their potential lyse environmental strains vitro at different MOIs temperatures; control bacterial community. Three distinct phages designated sww65, sww275, sww297; defined by plaque morphology, electron microscopy host range; obtained wastewater. Challenge tests performed 37, 30 °C with infection cultures individual phage, a mixture two phages, cocktail three 10 0 ,1 2 , 4 PFU/CFU. At 30, 37 °C, reduced all tested. These results required high multiplicity infection. However, when infected only one phage or PFU/CFU, emergence resistance was observed. The dynamic monitoring enterobacterial community conducted using Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). number bands decreased gradually use cocktails. Moreover, during treatment PCR detection virulence gene invA. correlated ERIC-PCR fingerprints, suggested that affected treatment. Indeed, wastewater, reducing other members Enterobacteriaceae. indicated changes closely related process introduction wide range can have impact on dynamics microbial communities, manifested reduction elimination species.