Microfabricated devices for biomolecule encapsulation

摘要: Biomolecule encapsulation in droplets is important for miniaturizing biological assays to reduce reagent consumption, cost and time of analysis, can be most effectively achieved by using microfabricated devices. Microfabricated fluidic devices generate emulsified drops uniform size with controlled dimensions contents. Biological chemical components such as cells, microgels, beads, hydrogel precursors, polymer initiators, other encapsulated within these drops. Encapsulated emulsions are appealing a variety applications since used tiny reaction vessels perform high-throughput reactions at fast rates, consuming minimal sample solvent amounts due the small (micron diameters) emulsion Facile mixing droplet coalescence allow diversity performed on-chip tunable parameters. The simplicity operation speed analysis microencapsulated lends itself well an array quantitative biomolecular studies directed evolution, single-molecule DNA amplification, single-cell encapsulation, sequencing, enzyme kinetics, microfluidic cell culture. This review highlights recent advances field encapsulating devices, emphasizing development emulsifying encapsulations, device design, current that droplets.