Spatial analysis of plant metabolism: sucrose imaging within Vicia faba cotyledons reveals specific developmental patterns.

摘要: *Summary During legume embryogenesis the differentiation of cotyledons proceeds gradually in a wave-like manner. The process is metabolically and genetically controlled regulated by sugars. In order to perform spatial temporal analysis sugar distribution pattern new method was developed specifically measure sucrose directly tissues via bioluminescence single photon counting. This enabled quantitative imaging with resolution close cell level. procedure applied on sections Vicia faba covering main stages histodifferentiation. Young embryos before storage phase contained moderate levels sucrose, which were evenly distributed. At onset maturation high concentrations present within tissue layer outwarded half coytledons. underneath epidermis expressing transporter gene indicating that epidermal transporters caused accumulation underlying tissue. stage gradient inversely oriented compared size starch content. Cells interior larger, but low sucrose. Thus, uptake activity permeability parenchyma. However, during actively elongating accumulating cells contain highest differences growth also affect intracotyledonary distribution. High correlated transcript synthase ADP-Glc pyrophosphorylase signaling function for induce biosynthesis expression Carbon flux through pathway towards increased when hexoses decreased.