Optimisation of glycogen quantification in mixed microbial cultures
作者： Ana B. Lanham , Ana R. Ricardo , Marta Coma , Joana Fradinho , Mónica Carvalheira
DOI: 10.1016/J.BIORTECH.2012.05.087
关键词:
摘要: This study addressed the key factors affecting extraction and quantification of glycogen from floccular granular mixed microbial cultures collected activated sludge, nutrient removal systems photosynthetic consortiums: acid concentration, hydrolysis time concentration biomass in hydrolysis. Response surface modelling indicated that 0.9 M HCl a 1 mg mL(-1) were optimal conditions for performing Floccular samples only needed 2-h whereas required as much 5 h. An intermediate 3 h yielded an error 10% compared to results obtained with times specifically tailored type can thus be recommended practical compromise.
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