ENUMERATION OF THERMALLY STRESSED Staphylococcus aureus MF 31

摘要: Procedures used to enumerate microorganisms in food should detect all viable organisms present, regardless of their physiological state. Several currently recognized procedures were unable satisfactorily thermally stressed cells Staphylococcus aureus. After heating at 52°C 100 mM potassium phosphate buffer pH 7.2, the enumerated by comparative platings on both selective and nonselective media a most probable number technique (MPN). All produced similar counts unheated cells. When heated plating media, productivity varied with degree stress imposed particular medium used. Baird-Parker's normal equally well. inoculated into trypticase soy broth + 10% NaCl plate count decreased approached that medium. an extended lag then increased parallel manner.