Regulatory T Cells Dynamically Regulate Selectin Ligand Function during Multiple Challenge Contact Hypersensitivity

摘要: Regulatory T cells (Tregs) play critical roles in restricting cell-mediated inflammation. In the skin, this is dependent on expression of selectin ligands required for leukocyte rolling dermal microvessels. However, whether there are differences molecules used by Tregs and proinflammatory to undergo skin remains unclear. study, we spinning disk confocal microscopy Foxp3-GFP mice visualize endogenous postcapillary venules. underwent consistent but low-frequency interactions under resting inflamed conditions. At early stage response, Treg adhesion was minimal. at peak inflammation, made up 40% adherent CD4(+) cell population. a multiple challenge model contact hypersensitivity, conventional mostly overlapping contributions P- E-selectin. after second challenge, not became P-selectin independent, showed reduced capacity bind P-selectin. Moreover, inhibition E-selectin time point resulted exacerbation These findings demonstrate that binding molecular basis can be regulated dynamically.