A novel recombinant papillomavirus genome enabling in vivo RNA interference reveals that YB-1, which interacts with the viral regulatory protein E2, is required for CRPV-induced tumor formation in vivo.

摘要: YB-1 is considered a negative prognostic marker for different types of cancer. Increased protein levels in tumor cells indicate worse prognosis. In preceding study comparing the transcripts CRPV-induced benign papillomas to mRNA malignant epithelial tumors, we identified as gene that up-regulated papillomavirus-associated carcinomas and which causes an invasive phenotype CRPV-positive vitro. Here demonstrate previously unknown factor required papillomavirus-induced development rabbit animal model system. By infecting animals with novel recombinant shRNA-expressing CRPV genome, show knock-down dramatically reduces papillomavirus-dependent formation vivo. Consistent previous reports showing nuclear distribution proteins hallmark malignancy, predominantly localization CRPV-immortalized cells. Furthermore give evidence regulating URR thereby viral expression interactor regulatory E2. Taken together hypothesize essential probably by including oncogenes E6 E7.