作者: Maria Vera , Jesus Prieto , David S. Strayer , Puri Fortes
DOI: 10.1016/J.YMTHE.2004.06.1014
关键词:
摘要: Most gene therapy approaches employ viral vectors for delivery. Ideally, these should be produced at high titer and purity with well-established protocols. Standardized methods to measure the quality of are imperative, as techniques that allow reproducible quantitation titer. We devised a series protocols achieve high-titer production purification provide control titering recombinant simian virus 40 (rSV40s). rSV40s good candidate vehicles transfer: they easily modified nonreplicative nonimmunogenic. Further, infect wide variety cells long-term transgene expression. report here produce rSV40 in yields, describe their purification, characterize stocks using monitor presence wild-type SV40 revertants defective interfering particles. Several titration viruses have been compared. believe can widely applied obtain concentrations high-quality reproducibly.