Chloroplast DNA phylogeography of the shrub Cistus ladanifer L. (Cistaceae) in the highly diverse Western Mediterranean region

摘要: This study investigated the phylogeographic structure of Cistus ladanifer, in order to locate its Quaternary refugia, reconstruct recolonisation patterns and assess role geographical features (mountain ranges, rivers Strait Gibraltar) as barriers seed flow expansion through Western Mediterranean. Thirty-eight populations were screened for length variation polymorphic chloroplast simple sequence repeats (cpSSRs). Statistical analyses included estimation haplotypic diversity, hierarchical analysis molecular (AMOVA) fixation indices. Mantel tests, SAMOVA BARRIER applied evaluate partitioning genetic diversity across entire species range. Pollen data from bibliography used complement inferences. Chlorotype within was similar throughout natural range C. ladanifer (mean = 0.32). High differentiation among estimated (GST 0.60). Our suggest that Gibraltar Betic ranges may have favoured divergence during glacial periods four different lineages inferred with SAMOVA. The main northward colonisation Iberian Peninsula occurred refugia southwest Iberia. process been influenced by human activities (forest clearance, livestock grazing even commerce) Peninsula. In contrast, area conserved a specific haplotype.