The Brain In Vivo Expresses the 2′,3′-cAMP-Adenosine Pathway

摘要: J. Neurochem. (2012) 122, 115–125. Abstract Although multiple biochemical pathways produce adenosine, studies suggest that the 2′,3′-cAMP-adenosine pathway (2′,3′-cAMP2′-AMP/3′-AMPadenosine) contributes to adenosine production in some cells/tissues/organs. To determine whether exists vivo brain, we delivered brain (gray matter and white separately) via inflow perfusate of a microdialysis probe either 2′,3′-cAMP, 3′,5′-cAMP, 2′-AMP, 3′-AMP, or 5′-AMP measured recovered metabolites outflow with mass spectrometry. In both gray matter, 2′,3′-cAMP increased 3′-AMP 3′,5′-cAMP adenosine. regions, 3-AMP were converted Microdialysis experiments 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) wild-type mice demonstrated traumatic injury (controlled cortical impact model) activated pathway; similar CNPase knockout indicated was involved metabolism endogenous 2′-AMP CSF from patients, significantly initial 12 h after strongly correlated levels inosine. We conclude vivo, is 2′-AMP/3′-AMP, these AMPs are metabolized This endogenously humans.