Thiol labeling of DNA for attachment to gold surfaces

摘要: A method for adsorbing nucleic acids to a surface of gold substrate analyzing the structure acid includes steps thiolating by substituting at least one non-bridging internucleotide oxygen each phosphodiester moiety with sulfur, depositing thiolated on surface, and subjecting analysis determining atomic or molecular thereof. The is prepared single crystal mechanical polishing, electropolishing, cleaning cycles Ar + sputtering annealing under vacuum until no contamination detected Auger electron spectroscopy, flame quenching in methanol. Gold films deposited atomically flat substrates, such as mica, can also be used. DNA sufficient time covalent bonds form between sulfur gold. Scanning tunneling microscopy, force angle-dependent x-ray photoelectron spectroscopy are used analyze structure.