Vertical Profiles Of Methanogenesis And Methanogens In Two Contrasting Acidic Peatlands In Central New York State, USA

摘要: Northern acidic peatlands are important sources of atmospheric methane, yet the methanogens in them poorly characterized. We examined methanogenic activities and methanogen populations at different depths two peatlands, McLean bog (MB) Chicago (CB). Both have (pH 3.5-4.5) peat soils, but pH deeper layers CB is near-neutral, reflecting its previous existence as a neutral-pH fen. Acetotrophic hydrogenotrophic methanogenesis could be stimulated upper samples from both bogs, phylotypes using H2/CO2 (Methanomicrobiales) or acetate (Methanosarcinales) were identified 16S rRNA gene clone libraries by terminal restriction fragment length polymorphism (T-RFLP) analyses novel primer/restriction enzyme set that we developed. Particularly dominant was clade Methanomicrobiales, called E2 here R10 fen group elsewhere, estimated quantitative polymerase chain reaction to present approximately 10(8) cells per gram dry peat. Methanogenic activity considerably lower bogs. The detected T-RFLP portions MB mainly uncultured euryarchaeal rice cluster (RC)-II group, whereas less deep different, included Methanomicrobiales call E1-E1', well RC-I, RC-II, marine benthic D, new subaqueous cluster. barely detectable CB, further evidence for associations most organisms this with habitats.