Regulation of interleukin 2 receptor expression: effects of phorbol diester, phospholipase C, and reexposure to lectin or antigen.

摘要: Anti-Tac monoclonal antibody identifies the receptor for interleukin 2 (IL 2, or T cell growth factor) present on activated human lymphocytes. By using tritiated anti-Tac, we now report a sensitive and specific binding assay to evaluate surface IL expression. receptors peripheral blood lymphocytes can be detected within 6 hr after PHA stimulation. PHA-induced expression is inhibited by actinomycin D cycloheximide, but not mitomycin C, suggesting requirement de novo RNA protein synthesis, DNA synthesis. Scatchard analysis of [3H]-anti-Tac stimulated with 3 days revealed from 20,000 60,000 molecules bound per cell, Kd 1 x 10(-10) mol/l. Sequential studies maintained in long-term culture demonstrated progressive decline number correlating diminished rate. Restimulation lectin antigen increased receptors, that dependent immune responses may regulated, at least part, Receptor was also PMA. Moreover, similar effects were produced incubation phospholipase C 1. Because both PMA result activation kinase these data suggest possibility induce