The Establishment of Vesicular-Arbuscular Mycorrhiza under Aseptic Conditions

摘要: SUMMARY: The establishment of vesicular-arbuscular mycorrhizal infections by inoculation with germinated resting spores an Endogone sp. was investigated under microbiologically controlled conditions; pure two-membered cultures were obtained for the first time. Seedlings grown in a nitrogen-deficient inorganic salt medium; these conditions fungus failed to form appressorium and penetrate plant roots unless Pseudomonas also added. Adding soluble nitrogen medium completely inhibited root penetration, even presence bacteria. Various sterile filtrates could be used replace bacterial inoculum but substitutes induced only few per plant. Mycorrhizal grew more vigorously than non-mycorrhizal same seedling. They longer profusely branched. At predominantly arbuscular, many prominent vesicles developed as seedlings declined, then out infected colonized agar. not subcultured without living host. The possible interpretation results is considered reference specialized nutritional which test plants grown.