Microsatellite-based genetic profiling for the management of wild and captive flamingo populations.

摘要: Flamingo species generate tremendous interest whether they are small captive groups or wild populations numbering in the thousands. Genetic pedigrees invaluable for maintaining maximum genetic diversity captive, as well wild, populations. However, presently there is a general lack of data flamingo Microsatellites loci composed 2-6 base pair tandem repeats, scattered throughout higher eukaryotic genomes, often exhibiting high levels polymorphism and heterozygosity. These thus important markers identity, parentage population studies. Here, six microsatellite were isolated from microsatellite-enriched Caribbean partial genomic library. Two compound complex repeats four perfect trinucleotide repeats. Each locus was amplified Caribbean, African greater, Chilean lesser DNAs. Heterozygosity frequencies calculated (range 0.12-0.90) greater flamingos 0.23-0.94) loci. All found to be Hardy-Weinberg equilibrium linkage disequilibrium analyses did not suggest any two subspecies (African Caribbean) At least five also exhibit flamingos, but due sample numbers, relevant allele/heterozygosity frequency calculations could estimated. Nucleotide sequence comparisons amplicons derived reveal level conservation at all Although numbers again limit some degree birds, sequences identical number nonconserved nucleotides appears lesser/greater than Chilean/greater comparisons. This consistent with being different within same genus while belong separate genus. Parentage on suggested family Disney's Animal Kingdom's collection performed using data. Results confirmed many other cases one more parents clearly excluded. The provide new management tool useful both