Synergistic killing effect of chloroquine and androgen deprivation in LNCaP cells

摘要: Modulation of autophagy is a new paradigm in cancer therapeutics. Recently novel function chloroquine (CLQ) inhibiting degradation autophagic vesicles has been revealed, which raises the question whether CLQ can be used as an adjuvant targeting pro-survival mechanism prostate (PCa). We previously showed that played protective role during hormone ablation therapy, part, by consuming lipid droplets PCa cells. In addition, blocking genetic and pharmacological means presence androgen deprivation caused cell death To further investigate importance survival dissect death, we treated responsive LNCaP cells with combination deprivation. observed synergistically killed dose- time-dependent manner. confirmed inhibited maturation decreased cytosolic ATP. Moreover, induced nuclear condensation DNA fragmentation, hallmark apoptosis, deprived Taken together, our finding suggests may useful therapy to improve therapeutic efficacy.