Long-Term Intake of a High-Protein Diet with or without Potassium Citrate Modulates Acid-Base Metabolism, but Not Bone Status, in Male Rats

摘要: High dietary protein intake generates endogenous acid production, which may adversely affect bone health. Alkaline potassium citrate (Kcit) contribute to the neutralization of protein-induced metabolic acidosis. We investigated impact 2 levels and Kcit supplementation on acid-base metabolism status in rats. Two-monthold Wistar male rats were randomly assigned 4 groups (n ¼ 30 per group). Two received a normal-protein content (13%) (NP) or high-protein (HP) diet (26%) for 19 mo. The other identical diets supplemented with (3.60%) (NPKcit HPKcit). Rats pair-fed based ad libitum HP group. At 9, 16, 21 mo age, 10 each group killed. induced acidosis characterized by hypercalciuria, hypermagnesuria, hypocitraturia at all ages. neutralized this effect, as evidenced decreased urinary calcium magnesium excretion HPKcit Femoral mineral density, biomechanical properties, biomarkers (osteocalcin deoxypyridinoline), plasma insulin-like growth factor 1 not affected different diets. Nevertheless, retention was reduced This study suggests that lifelong excess results low-grade without affecting skeleton, be protected an adequate supply. J. Nutr. 138: 718–724, 2008.