Coronavirus nucleocapsid protein is an RNA chaperone.

摘要: RNA chaperones are nonspecific nucleic acid binding proteins with long disordered regions that help molecules to adopt its functional conformation. Coronavirus nucleoproteins (N) RNA-binding regions. Therefore, we investigated whether transmissible gastroenteritis coronavirus (TGEV) N protein was an chaperone. Purified enhanced hammerhead ribozyme self-cleavage and acids annealing, which properties define chaperones. In contrast, another protein, PTB, did not show these activities. chaperone activity blocked by specific monoclonal antibodies. it concluded TGEV is addition, have shown purified severe acute respiratory syndrome (SARS)-CoV also has activity. silico predictions of domains showed a similar pattern for all evaluated. Altogether, data led us suggest might be