Antibody-dependent, cell-mediated cytolysis (ADCC) with antibody-coated effectors: rat and human effectors versus tumor targets.

摘要: We have previously described techniques that cause antibody molecules to remain bound P388D1 cells for at least 18 hr, and enable these lyse hapten-coated erythrocytes not sensitized with antibody. These methods collectively are called "franking." In this study, we determined applicable other systems. franked rat splenocytes human peripheral blood leukocytes rabbit anti-TNP antibody, showed they were capable of lysing TNP-tumor erythrocyte targets (not coated antibody) in a hapten-specific, antibody-dependent fashion. Both the mononuclear polymorphonuclear (PMN) leukocyte fractions mediating lysis. Additionally, stained fluorescent goat anti-rabbit IgG Fab, analyzed fluorescence by flow microfluorometry. Nearly all PMN about one-half had on their surfaces after franking. Clearly, can be franked, but those retain significant numbers (approximately 5 X 10(4), case cells) surfaces.