Elucidation of cellular targets responsible for tetrachlorodibenzo-p-dioxin (TCDD)-induced suppression of antibody responses: II. The role of the T-lymphocyte.

摘要: Various immunological assays have been applied by our laboratory in an attempt to assess the role of T-cell TCDD-induced suppression antibody response murine B6C3F1 splenic lymphocytes. Animals were treated vivo (via gavage) with 1.0 microgram/kg TCDD corn oil for 5 days before vitro analysis splenocyte immunocompetence and function. To study effects on T-helper cell function, alterations proliferative responses T-cells following exposure investigated. Results show no significant difference [3H]thymidine uptake between vehicle- TCDD-treated whole splenocytes 24 h after stimulation mitogen Con A. This is consistent finding that IL-2 production at either or 48 A lymphocytes was not significantly different from vehicle-treated controls. The possibility induction a suppressor also Titration mice into naive cultures did suppress humoral T-dependent (SRBC) T-independent (DNP-Ficoll) antigen. In contrast, titration cells stimulated (a positive control T-cell) inhibited both types Likewise, plus macrophages responsiveness B-cells antigen (DNP-Ficoll). These results would indicate alteration function does play elicited splenocytes.