Scanning Electron Microscope Analysis of Stromal Surface Regularity in DSAEK Using Manual or Automated Microkeratomes or DMEK.

摘要: PURPOSE To analyze differences in the stromal bed according to method used obtain disc for endothelial transplant with 1) an automated microkeratome Descemet stripping keratoplasty (DSAEK), 2) homogeneous dissection of membrane (Descemet [DMEK]), or 3) a manual DSAEK. METHODS We analyzed 25 consecutive corneas transplantation, divided into 3 groups: 11 samples cut microkeratome, 5 DMEK, 9 microkeratome. A scanning electron microscope was image center bed. The irregularity index surface complementary obtained grafting calculated Canny algorithm (0: completely smooth; 1: irregular). RESULTS At all thresholds studied (20, 30, 50, and 70), mean group C (35.2, 24.4, 13.7, 8.8, respectively) higher than that (26.2, 14.8, 6.7, 4.0, respectively), which turn B (7.0, 4.2, 2.4, 1.8, respectively). Differences were statistically significant among groups thresholds. CONCLUSIONS Irregularity after any can be quantified using method. use DSAEK reduces helps more similar DMEK