Spectroscopic techniques for photodynamic therapy dosimetry

摘要: Photodynamic therapy (PDT) as a cancer treatment modality relies on the simultaneous presence of three components; light, photosensitiser and oxygen. Once excited by can interact with oxygen, leading to formation toxic oxygen species. These reactive substances induce cellular damage within irradiated tissue volume. PDT has been investigated for treating malignancies in numerous organs is nowadays an approved certain types of, example, skin, lungs, bladder oesophagus. However, still suffers from several drawbacks. For many photosensitisers accumulate also non-malignant tissue, introducing risk damaging surrounding, sensitive tissue. Another drawback large intra- inter-patient variation response despite utilising standardised light doses. In this thesis, two approaches have aim overcome one hand, sub-optimal tumour-selective uptake photosensitiser, other, variable effects. both tasks, spectroscopic techniques were employed monitor parameters relevant effect. The first project involved pharmacokinetic studies novel, liposomal formulation topical intravenous administration. The application route led good animal skin tumour model human malignancies. However, clinical conditions, such time, doses, need be further optimised. Furthermore, following systemic administration murine model, pharmacokinetics novel rapid biodistribution clearance blood stream. tumour-to-skin tumour-to-muscle selectivity at eight hours after higher than reported free mTHPC formulation. The second approach aimed implementing realtime feedback interstitial PDT. series instruments incorporated six optical fibres delivery monitoring transmission fluorescence target Post-treatment data analysis indicated significant differences between intended actual dose distributions. Hence, we concluded there order ascertain prescribed entire More recently, hardware adapted prostate. number source increased 18 based threshold implemented. We now present instrument prostate incorporating modules pre-treatment planning, properties updating irradiation times realtime. This scheme evaluated simulated scenarios which it was that dosimetry module makes possible deliver spatial temporal variations