Release of bioactive peptides from polyurethane films in vitro and in vivo: Effect of polymer composition

摘要: Abstract Thermoplastic polyurethanes (TPUs) are widely used in biomedical applications due to their excellent biocompatibility. Their role as matrices for the delivery of small molecule therapeutics has been reported. However, very little is known about release bioactive peptides from this class polymers. Here, we report linear and cyclic TPUs with different hard soft segments. Solvent casting TPU at room temperature mixed resulted reproducible efflux profiles no evidence drug degradation. Peptide was dependent on size well composition TPU. Tecoflex 80A (T80A) showed more extensive than ElastEon 5-325, which correlated a degree hydration. It also shown that medium influenced rate extent peptide efflux. Blending allowed better control efflux, especially initial burst effect. Peptide-loaded prolonged plasma levels anti-inflammatory PMX53, normally half-life less 30 min. Using blend T80A E5-325, therapeutic PMX53 were observed up 9 days following single intraperitoneal implantation drug-loaded film. released blended significantly inhibited B16-F10 melanoma tumor growth mice demonstrating its bioactivity vivo . This study provides important findings TPU-based could improve pharmacological utility therapeutics. Statement Significance Therapeutic can be highly specific potent agents, but poorly absorbed rapidly degraded body. overcome by using matrix protects promotes slow so effect achieved over days or weeks. versatile family polymers biocompatible medical implants. several range depend type polymer polyurethane. first examine polyurethane blends shows fine-tuned segment mixtures polymer.