Astroglial and fibroblast growth factors have neurotrophic functions for cultured peripheral and central nervous system neurons

摘要: Abstract Embryonic and neonatal neurons require specific trophic supplements for their survival the induction of transmitter-synthesizing enzymes in vivo vitro. Acidic basic fibroblast growth factor (aFGF, bFGF) closely related astroglial factors AGF-1 AGF-2 were studied putative neurotrophic functions using dissociated, highly neuron-enriched cultures from chick rat peripheral ganglia central nervous system tissues. Embryonic ciliary ganglion only that responded to bFGF by enhanced equivalent obtained with factor. Half-maximal effects achieved at 360 pg/ml or 3 ng/ml. Small seen aFGF could be potentiated adding heparin 1 microgram/ml. bFGF, but not neurotropic factor, also promoted neuron after was bound polyornithine laminin. Both induced choline acetyltransferase activity during 48 hr. AGFs FGFs long-term embryonic spinal cord neurons, including motoneurons had been retrogradely labeled rhodamine isothiocyanate. These results demonstrate potency a class mitogenic as agents neurons--adding emerging evidence neuronal are strictly separate entities.