The role of CXCR4 signaling in the migration of transplanted oligodendrocyte progenitors into the cerebral white matter.

摘要: Enhancing the ability of either endogenous or transplanted oligodendrocyte progenitors (OPs) to engage in myelination may constitute a novel therapeutic approach demyelinating diseases brain. It is known that adults neural situated subventricular zone lateral ventricle (SVZ) are capable generating OPs which can migrate into white matter tracts such as corpus callosum (CC). We observed progenitor cells SVZ adult mice expressed CXCR4 chemokine receptors and SDF-1/CXCL12 was CC. therefore investigated role signaling regulating migration CC following their transplantation ventricle. established OP cell cultures from Olig2-EGFP mouse brains. These variety receptors, including receptors. differentiated CNPase-expressing oligodendrocytes culture. To study migratory capacity vivo, we them ventricles mice. Donor migrated mature oligodendrocytes. This enhanced animals with Experimental Autoimmune Encephalomyelitis (EAE). Inhibition receptor expression using shRNA inhibited suggesting directed regulated by signaling. findings indicate mediated important guiding context inflammatory brain disease.