Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements

摘要: This chapter describes in vitro methods for studying the blood-brain barrier. These include a cell line and isolated brain microvessels. The rat endothelial 4 (RBE4) express many properties that are expressed by cells vivo. Tissue culture allow investigator to design experiments transporters permeability would be much more difficult A method making preparations of microvessels also is described. highly enriched can used transport vitro, but their short life span limitation. Two discussed measuring culture. In one method, measured across mono-layer. useful luminal abluminal transport. second especially designed families efflux transporters. will complement vivo techniques, they may as screening timely expensive experiments, reducing need experimental animals.