Retrovirus packaging cells based on 10A1 murine leukemia virus for production of vectors that use multiple receptors for cell entry.

摘要: 10A1 murine leukemia virus can enter cells by using either of two different cell surface phosphate transport proteins, the gibbon ape receptor Glvr-1 (Pit-1) or amphotropic retrovirus Ram-1 (Pit-2). and are widely expressed in tissues, but relative amounts each highly variable. We have developed packaging lines based on to take advantage this dual utilization improve gene transfer rates somatic animals humans, which levels receptors not always known. Optimization Env expression vector allowed generation that produce helper-free titers up 10(7)/ml. By interference analysis, we found a pseudotype retroviral utilize for efficient entry into mouse, rat, human mouse rat cells. The efficiently enters Glvr-1, while is much less efficient. Thus, may be advantageous compared with standard because vectors produced use an additional entry. These will also useful further explore complicated pattern usage conferred viral protein.