Influence of handling conditions on the establishment and propagation of head and neck cancer patient derived xenografts.

摘要: Background Patient derived xenografts (PDXs) for head and neck cancer (HNC) other cancers represent powerful research platforms. Most groups implant patient tissue into immunodeficient mice immediately although the significance of this time interval is anecdotal. We tested hypothesis that from tumor excision to implantation crucial PDX passaging establishment. Methods We examined whether or storage medium affected viability two established HNC PDXs (UW-SCC34, UW-SCC52). Tumors were harvested, stored in ice-cold media saline 0–48 hours, implanted new mice. Tumor growth was compared by two-way ANOVA with respect condition. Three (UW-SCC63-65) generated implanting (Time 0) 24 hours after receiving operating room. Results Similar quantities each mouse. At end experiment, no significant difference seen mean weight between conditions UW-SCC34 UW-SCC52 (p = 0.650 p 0.177, respectively). No formation prevalence on basis harvest (≥13 16 tumors grew at every point). Histological analysis showed strong similarity initial across all groups. developed both Time 0 UW-SCC63 UW-SCC64. Conclusions demonstrated neither nor (up 48 hours) histological differentiation a subsequent passage PDXs. Moreover, we revealed fresh viable up post-resection. This information important as it applies development sharing