Distribution Pattern and Diversity Maintenance Mechanism of the Denitrifier Community in Alkaline Copper Mine Drainage

摘要: ABSTRACT Denitrifiers have been shown to vary in abundance and diversity across the environmental gradients; however, factors that determine type magnitude of response denitrifier communities remain unclear. In order reveal effects alkaline mine drainage (AlkMD) on community composition maintenance mechanism a tailings reservoir, we examined patterns adaptation mechanisms different Shibahe copper water Zhongtiaoshan, China. The results showed α-diversity Proteobacteria nosZI-bearing downstream seeping (SDSW) were highest, while those upstream (STW1) lowest. highest nirS nirK-bearing measured STW1, lowest STW3. dissimilarity was strongly correlated with environment parameters, but varied groups. nitrate (NO3-); pH, total carbon (TC), NO3- ammonia nitrogen (NH4+), organic (TOC), dissolved oxygen (DO), NO3-, electroconductibility (EC) spatial distance (PCNM1). Our indicated changes valuable index reflecting variations AlkMD. Additionally, findings this study are helpful understanding relationship between microbial functional ecosystems. IMPORTANCE Metal pond is an important site polluted by heavy metals nitrogen, excess mainly removed microorganisms through denitrification. Although many studies reported main denitrifying bacteria, nirS, nirK nosZ-bearing denitrifiers three key groups denitrification process. However, less known their discharge wastewater (AlkMD). AlkMD exerts adverse impact structure bacteria community. change concentration along flow direction resulted pattern, say, filtering force drove highly ecosystem.