Cynandione A from Cynanchum wilfordii protects cultured cortical neurons from toxicity induced by H2O2, L-glutamate, and kainate.

摘要: Oxidative stress has been implicated as a primary cause of neuronal death in certain neurodegenerative disorders and aging brains. Natural products have used Asian societies for centuries treating such senile dementia. In an effort to identify active neuroprotective compounds from these products, we employed cultures rat cortical neurons our screening system. A methanolic extract dried roots Cynanchum wilfordii Hemsley (Asclepiadaceae) significantly mitigated the neurotoxicity induced by H2O2 this Activity-guided fractionation using several chromatographic techniques resulted isolation compound, cynandione A, biacetophenone. At concentration 50 microM, reduced H2O2. Cynandione attenuated decreases levels glutathione, superoxide dismutase, other enzymes that participate cellular defense against oxidative stress. Furthermore, alleviated excitotoxic neurotransmitter, L-glutamate, kainate, but not mediated N-methyl-D-aspartate. was demonstrated be natural antioxidant it facilitated breakdown hydrogen peroxide vitro; however, no mechanism uncovered explain its neuroprotectant effects glutamate kainate. Therefore, may efficacious protecting via activation alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate/kainate receptors since exerted significant on cultured neurons.