Protein fractionation in a multicompartment device using Off-Gel™ isoelectric focusing

摘要: A new protein fractionation technique based on off-gel isoelectric focusing (IEF) is presented, where the proteins are separated according to their point (pI) in a multiwell device with advantage be directly recovered solution for further analysis. The fractions obtained this have then been characterized polymer nanoelectrospray mass spectrometry (MS) analyses or Bioanalyzer identification. This methodology shows possibility of developing alternatives classical two-dimensional (2-D) gel electrophoresis. One species numerical simulation electric field distribution during separation also presented order demonstrate principle purification. Experiments pI markers carried out highlight kinetics and efficiency technique. Moreover, resolution was shown 0.1 pH unit -lactoglobulin B. In addition, an Escherichia coli extract performed standard solubilization buffer performances technique, notably proteomics applications.