作者: K.S. Schumaker , H. Sze
DOI: 10.1016/S0021-9258(18)61292-X
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摘要: In plant cells, transient changes in cytoplasmic Ca2+ levels can modulate numerous developmental processes. is accumulated the vacuole via a H+/Ca2+ antiport system that energized by tonoplast H+-pumping ATPase. Inositol 1,4,5-triphosphate (InsP3), but not inositol 1,4-bisphosphate, myo-inositol 1-phosphate, or fructose 2,6-bisphosphate, caused reduction of vesicles. The decrease was dependent on InsP3 concentration (Km apparent = 0.6 microM). InsP3-induced release blocked antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl. These results suggest vacuolar membrane one target site for action and may operate as second messenger mobilization intracellular cells.