Growth Inhibition, Enhancement of Intercellular Adhesion, and Increased Expression of Carcinoembryonic Antigen by Overexpression of Phosphoinositides-specific Phospholipase C β1 in LS174T Human Colon Adenocarcinoma Cell Line
作者： Koji Nomoto , Naohiro Tomita , Masami Miyake , Ding-Bang Xhu , Paul R. LoGerfo
DOI: 10.1111/J.1349-7006.1998.TB00522.X
关键词:
摘要: By using a retrovirus-derived system we generated derivatives of the human colon adenocarcinoma cell line LS174T (ATCC CL 188) that stably overexpress full-length cDNA encoding beta 1 isoform bovine phosphoinositides-specific phospholipase C (PI-PLC). This was confirmed by elevated levels catalytic activity to release phosphoinositides from phosphatidylinositol (PI-PLC) or phosphatidylinositol-bis-phosphate (PIP2-PLC), and enhanced expressions messenger RNA protein. PI-PLC overexpresser clones grew form clumps floating in liquid medium, whereas pMV7-introduced control displayed morphologic characteristics were very similar those parent line. Three individual lines increased doubling time (18.0 h, 21.5 23.8 h) when compared with 4 (13.1 10.7 12.9 9.3 h). Anchorage-independent growth ability soft agar medium dramatically suppressed overexpression PLC 1, PLC-overproducer aggregates cultured clones. Tumorigenicity 1-overproducers much weaker than vector-transduced The spontaneous carcinoembryonic antigen 1-overproducer higher pMV7 during suspension culture stronger These results provide first evidence endogenous suppress tumor growth, but enhance antigen, an intercellular adhesion molecule.
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