Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: the role of solvent and hydroxyapatite particles.

摘要: Polyurethane (PU) is a promising polymer to support bone-matrix producing cells due its durability and mechanical resistance. In this study two types of medical grade poly-ether urethanes Z3A1 Z9A1 PU-Hydroxyapatite (PU-HA) composites were investigated for their ability act as scaffold tissue engineered bone. PU dissolved in varying concentrations dimethylformamide (DMF) tetrahydrofuran (THF) solvents electrospun attain scaffolds with randomly orientated non-woven fibres. Bioactive polymeric composite created using 15 wt% 70/30 DMF/THF solution incorporating micro- or nano-sized HA particles ratio 3:1 respectively, whilst 25 was doped 5:1. Chemical properties the resulting evaluated by FTIR physical SEM. Tensile testing carried out on all scaffolds. MLO-A5 osteoblastic mouse human embryonic mesenchymal progenitor cells, hES-MPs seeded test biocompatibility mineralised matrix production over 28 day culture period. Cell viability assayed MTT calcium collagen deposition Sirius red alizarin respectively. SEM images both PU-HA showed differences fibre morphology changes solvent combinations size particles. Inclusion THF eliminated presence beads fibres that present fabricated 100% DMF solvent, resulted more uniform thicker diameters. Mechanical demonstrated Young׳s Modulus yield strength lower at higher concentrations. sizes solutions reinforced leading properties, characterisation confirmed Although supported proliferation cell calcified matrix, containing nano-HA enabled highest deposition. These have potential bone formation engineering.