Purification of an Elicitor-Inducible Antifungal Chitinase from Suspension-Cultured Rice Cells
作者： R. Velazhahan , R. Samiyappan , P. Vidhyasekaran
DOI: 10.1007/BF02981742
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摘要: Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured were treated with elicitor isolated fromRhizoctonia solani, sheath blight pathogen. A fivefold increase in was observed 24 h after treatment. The elicitor-inducible purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa it has isoelectric point 8.3. basic inhibited mycelial growth ofR. solani vitro. Morphological changes appeared within 1 following exposure mycelium to chitinase. hyphal tip marked swelling subsequently lysis also observed.
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