Growth Dynamics of Fetal Human Neural Stem Cells

摘要: Human neural stem cells (hNSC) are useful for understanding neurogenesis, migration, other events in development, drug screening of developmental and disease targets, potential neurological therapeutics. These uses require expansion isolated hNSC culture with retention multipotency the ability to interact pathological environment a host. Growth fetal-derived non-immortalized, undifferentiated chemically defined serum-free media on bare “tissue culture-grade” polystyrene laminin-coated was measured understand critical growth parameters. On uncoated plastic, survival proliferation required (1) seeding freshly dispersed within specific range surface area density (2) allowing seed 3–5 days initial undisturbed growth. Cells grew stereotypical time course multilayer pattern characterized by extensive interactions both between surface. Laminin relaxed requirement promoted monolayer Analysis harvested cell yield per seeded as function revealed that laminin did not increase rate. Number seeded, density, were greatest predictors. Biphasic relations size suggest positive negative fetal influencing